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SEQUiT! in brief
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SEQUiT 4.1 - de novo peptide sequencing software is ...
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... precise
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in computation of complete peptide sequences |
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in successful sequence computing from tandem mass spectrometry data using even incomplete b- or y-fragment series |
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in accurate sequencing of tryptic and nontryptic (e.g. MHC peptides) peptides |
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in sequencing of chemically or post-translationally modified peptides |
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in different scoring systems for ESI- and MALDI-MS techniques (already successfully tested with data provided by MALDI-QTOF, MALDI-TOF (Post Source Decay), MALDI-TOF/ TOF (Laser Induced Dissociation, Collision Induced Dissociation), ESI-TRAP, ESI-QTOF and ESI-FTICR) |
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... highly customizable
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in extending individual amino acid list |
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in extendending own list of modifications |
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in using BLAST search engine in combination with different databases and taxonomy restrictions by GI (GenBank identifier) lists |
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... highly automated
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SEQUiT! supports common MS/MS data formats (e.g. *.sqi, *.mgf, *.dta, *.pkl) |
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You can process batch data: de novo sequencing with subsequent database searches |
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SEQUiT! generates report files with batch analysis results |
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... simple
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SEQUiT 4.1 allows intuitionally and uncomplicated usage.
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Examples: Screenshots
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File Manager
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De novo sequencing module
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Database Search (BLAST)
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Result Overview
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Results in Detail
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References
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SEQUiT! software Demine R., Walden P. Sequit: software for de novo peptide sequencing by matrix-assisted laser desorption/ionization post-source decay mass spectrometry. Rapid Commun Mass Spectrom. 2004; 18: 907-913.
Used in Demine R., Walden P. Testing the role of gp96 as peptide chaperone in antigen processing. J Biol Chem. 2005; 280: 17573-17578.
Hardware/software configuration Minimal: Pentium 4 CPU 1.70 MHz, 512 MB RAM, Windows 2000 or Windows XP, IE 6.0 Optimal: Pentium 4 CPU 3.40 MHz, 2 GB RAM, Windows 2000 or Windows XP, IE 6.0
Pentium is a registered trademark of Intel Corporation. Windows 2000 and Windows XP are registered trademarks of Microsoft Corporation
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(C) 2006 - Proteome Factory AG - All rights reserved |
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