Professional Protein Sequencing Service |
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N-terminal and internal protein sequencing
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Proteome Factory performs N-terminal protein and peptide sequencing by using an ABI Procise Model 491 Edman Micro Sequencer which is online connected to an ABI Model 140C PTH Amino Acid Analyzer. |
N-terminal protein sequencing using a Procise Edman Sequencer |
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Fee-for-service: N-terminal sequencing
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Proteome Factory offers N-terminal sequencing for proteins and peptides. The samples can either be lyophilized, in solution or blotted on to PVDF membrane. Depending on sample amount, amino acid sequence and customer’s needs 5 to about 40 amino acid residues can be N-terminally sequenced. |
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Fee-for-service: N-terminal protein sequencing
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Internal protein sequencing is offered as fee-for-service. Protein samples can either be lyophilized, in solution, in gel bands or blotted on PVDF membrane. The internal sequence analysis provides high-valued information for sequence analysis and characterization of complete proteins. In addition it is often performed if sequence information of N-terminally blocked proteins are required. |
Internal protein sequencing Internal sequencing of proteins is performed after enzymatic or chemical protein cleavage and reverse phase separation and fractionation of the generated peptides followed by N-terminal peptide sequencing. |
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