Service - Quality Control of Proteins

Proteome Factory offers the following standard analytical service portfolio for quality control of peptides, recombinant proteins, protein drugs and monoclonal antibodies.
Please contact us, if you need specific other protein analytical methods, advanced protein analysis, the development of new methods and techniques to fulfil your requirements.

Determination of molecular weight of proteins

Analysenmethode   Zielsetzung   Nettopreis pro Analyse (zzgl. 19% MwSt.)  
nanoESI-MS  Determination of molecular weight   200 EURO  
MALDI-MS  Determination of molecular weight up to ca. 100 kDa and more (on request)  120 EURO  
MS analysis of monoclonal antibody  Determination of molecular weight of the light and heavy chain  300 EURO 
FT-ICR-ESI-MS   High resolution determination of molecular weight (accuracy in low ppm range)  On request 
SDS-PAGE  Determination of protein mass by using MW standard proteins in SDS-PAGE   100 EURO  


Determination of amino acid sequence of proteins

Analysenmethode   Zielstellung   Nettopreis pro Analyse (zzgl. 19% MwSt.)  
N-terminal sequencing   Analysis of the N-terminal amion acid sequence and homogenity of the N-terminus  200 EURO (for 5 steps); additional steps: 40 EURO  
Protein sequencing  Analysis and validation of the primary amino acids sequence of complete proteins by mass spectrometry as well as N-terminal and internal Edman protein sequencing  On request 
Protein identification and peptide mapping  Conformation of protein identity and detectable peptides by nanoLC-ESI-MSMS after tryptic protein digest  275 EURO (incl. tryptic digestion and nanoLC-ESI-MSMS analysis) 
Analysis of disulfid bridges  Conformation and determination of the position of disulfid bridges  On request 
Analysis of post-translation modifications  Detection and conformation of sequence position of post-translational modification  On request 


Determination of purity and impurities

Analysenmethode   Zielsetzung   Nettopreis pro Analyse (zzgl. 19% MwSt.)  
2D electrophoresis, SDS-PAGE, western blot, MeCAT  Determination of homogenity and purity of a product or batch; detection and quantfication of isoforms and protein species   On request 
2D electrophresis and protein identification  Determination of homogenity and purity of a product or batch as well as identification of proteins, protein contaiminations and degradation products  On request 
Chromatography  Determination of homogenity and purity of protein and peptide products or batches  On request 
Bradford assay for protein concentration determination  Determination of protein concentration in a product or batch  On request 
MeCAT protein quantification by MeCAT labeling and ICP-MS  Absolute ultra sensitive and exact protein quantification in a product or batch  On request