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Service - Quality Control of Proteins

Proteome Factory offers the following standard analytical service portfolio for quality control of peptides, recombinant proteins, protein drugs and monoclonal antibodies.
Please contact us, if you need specific other protein analytical methods, advanced protein analysis, the development of new methods and techniques to fulfil your requirements.

Determination of molecular weight of proteins

Analysenmethode Zielsetzung Nettopreis pro Analyse (zzgl. 19% MwSt.)
nanoESI-MS Determination of molecular weight 200 EURO
MALDI-MS Determination of molecular weight up to ca. 100 kDa and more (on request) 120 EURO
MS analysis of monoclonal antibody Determination of molecular weight of the light and heavy chain 300 EURO
FT-ICR-ESI-MS High resolution determination of molecular weight (accuracy in low ppm range) On request
SDS-PAGE Determination of protein mass by using MW standard proteins in SDS-PAGE 100 EURO


Determination of amino acid sequence of proteins

Analysenmethode Zielstellung Nettopreis pro Analyse (zzgl. 19% MwSt.)
N-terminal sequencing Analysis of the N-terminal amion acid sequence and homogenity of the N-terminus 200 EURO (for 5 steps); additional steps: 40 EURO
Protein sequencing Analysis and validation of the primary amino acids sequence of complete proteins by mass spectrometry as well as N-terminal and internal Edman protein sequencing On request
Protein identification and peptide mapping Conformation of protein identity and detectable peptides by nanoLC-ESI-MSMS after tryptic protein digest 275 EURO (incl. tryptic digestion and nanoLC-ESI-MSMS analysis)
Analysis of disulfid bridges Conformation and determination of the position of disulfid bridges On request
Analysis of post-translation modifications Detection and conformation of sequence position of post-translational modification On request


Determination of purity and impurities

Analysenmethode Zielsetzung Nettopreis pro Analyse (zzgl. 19% MwSt.)
2D electrophoresis, SDS-PAGE, western blot, MeCAT Determination of homogenity and purity of a product or batch; detection and quantfication of isoforms and protein species On request
2D electrophresis and protein identification Determination of homogenity and purity of a product or batch as well as identification of proteins, protein contaiminations and degradation products On request
Chromatography Determination of homogenity and purity of protein and peptide products or batches On request
Bradford assay for protein concentration determination Determination of protein concentration in a product or batch On request
MeCAT protein quantification by MeCAT labeling and ICP-MS Absolute ultra sensitive and exact protein quantification in a product or batch On request