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SEQUiT! in brief

SEQUiT 4.1 - de novo peptide sequencing software is ...

... precise

in computation of complete peptide sequences
in successful sequence computing from tandem mass spectrometry data using even incomplete b- or y-fragment series
in accurate sequencing of tryptic and nontryptic (e.g. MHC peptides) peptides
in sequencing of chemically or post-translationally modified peptides
in different scoring systems for ESI- and MALDI-MS techniques (already successfully tested with data provided by MALDI-QTOF, MALDI-TOF (Post Source Decay), MALDI-TOF/ TOF (Laser Induced Dissociation, Collision Induced Dissociation), ESI-TRAP, ESI-QTOF and ESI-FTICR)

... highly customizable

in extending individual amino acid list
in extendending own list of modifications
in using BLAST search engine in combination with different databases and taxonomy restrictions by GI (GenBank identifier) lists

... highly automated

SEQUiT! supports common MS/MS data formats (e.g. *.sqi, *.mgf, *.dta, *.pkl)
You can process batch data: de novo sequencing with subsequent database searches
SEQUiT! generates report files with batch analysis results

... simple

SEQUiT 4.1 allows intuitionally and uncomplicated usage.

Examples: Screenshots

File Manager

De novo sequencing module

Database Search (BLAST)

BLAST (Basic Local Alignment Search Tool) is provided by National Center for Biotechnology Information (Bethesda, USA, www.ncbi.nlm.nih.gov) and is not a part of Sequit! software.